Laboratory for Breast Cancer Metastasis
Using single-cell technologies to unlock the cellular and molecular mechanisms driving metastasis
Single-cell genomics
We use diverse single-cell genomic and spatial technologies to study cellular heterogeneity and generate new insights into the basic cellular and molecular mechanisms that drive tissue homeostasis and metastasis.
Metabolism
Cells use different forms of metabolism depending on their bioenergetic needs and access to nutrients. We study the role of cellular metabolism in metastatic spread and why specific forms of metabolism are beneficial for metastasis.
Immune control
We study how the immune system controls metastasis to the central nervous system (CNS), a particularly deadly form of metastasis. The CNS immune microenvironment is unique and its ability to control metastasis is poorly understood.
HBCA
The goal of this project is to generate a comprehensive reference of cell types and cell states in the adult human breast tissues using single cell and spatial genomic methods. This is part of the greater Human Cell Atlas (HCA) effort and is generously funded by the Chan-Zuckerberg Initiative (CZI).
Featured publication
Transcriptional diversity and bioenergetic shift in human breast cancer metastasis revealed by single-cell RNA sequencing
Davis RT et al., Nature Cell Biology, 2020
Although metastasis remains the cause of most cancer-related mortality, mechanisms governing seeding in distal tissues are poorly understood. Here, we establish a robust method for the identification of global transcriptomic changes in rare metastatic cells during seeding using single-cell RNA sequencing and patient-derived-xenograft models of breast cancer. We find that both primary tumours and micrometastases display transcriptional heterogeneity but micrometastases harbour a distinct transcriptome program conserved across patient-derived-xenograft models that is highly predictive of poor survival of patients. Pathway analysis revealed mitochondrial oxidative phosphorylation as the top pathway upregulated in micrometastases, in contrast to higher levels of glycolytic enzymes in primary tumour cells, which we corroborated by flow cytometric and metabolomic analyses. Pharmacological inhibition of oxidative phosphorylation dramatically attenuated metastatic seeding in the lungs, which demonstrates the functional importance of oxidative phosphorylation in metastasis and highlights its potential as a therapeutic target to prevent metastatic spread in patients with breast cancer.
Devon A. Lawson, PhD
